RMBL Non-Spatial Metadata Owner and Metadata Title:   Karl Grunseich  <div>Stream Invertebrate Excretion 2009-- widths for NH4 Uptake <br> </div>



RMBL non-spatial metadata record ID #488
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Project Contact Information
Head PI /OwnerKarl Grunseich Affiliation: REU
Project Title: 
Stream Invertebrate Excretion 2009-- widths for NH4 Uptake
Data Collector:  Karl Grunseich Email: karl.grunseich@dartmouth.edu
Metadata Collector:  Karl Grunseich Email: karl.grunseich@dartmouth.edu

Project Classification Information
Level: ecosystem Theme:  Research Subject: other
Project:  Experimental Location:  other
Organism 1:  Stream Invertebrates Organism 2:  Stream Invertebrates

Project Information
Project keywords: Nutrient Cyclying, Excretion Rate, Stream Invertebrates, Ephemeroptera, Diptera, Plecoptera, Trichoptera
Project purpose:  The purpose of this study is to examine differences among taxa in excretion rate and to determine what percent of nutrient demand is met by invertebrate excretion.
Project methodology:  
I measured uptake rates of N using short-term, whole-stream additions of NH4 following standard methods (Stream Solute Workshop 1990, Mulholland et al. 2002). I added low levels of NH4Cl and a conservative tracer dye (Rhodamine WT) into the stream and monitored the concentration of the dye 600 m downstream every 2 minutes with a submersible fluorometer. When the concentration of Rhodamine dye reached an equilibrium concentration (~1 hour), I sampled nutrient concentrations at 50-75 m intervals along the stream section. Per meter nutrient uptake rate was estimated from the longitudinal decline in nutrient concentrations along the 600 m reach. I used the per meter nutrient uptake estimates to calculate areal uptake rates, or ecosystem demand, for N following the equations in the Stream Solute Workshop (1990). 

Project abstract:  
Nutrient supply into an ecosystem is an important factor that can influence its productivity. While some nutrients enter the ecosystem from external sources, cycling of nutrients internally can also be an important process in determining nutrient supply. In this study I examined the role of benthic stream macroinvertebrates in supplying nutrients back into the water column. I measured excretion rates of NH4-N for all readily identifiable taxa in the upper East River in Gothic, CO and examined how these rates varied by body size and across taxa. I also measured nutrient uptake rates in order to determine what percent of ecosystem demand is met by the macroinvertebrate community.  I found that invertebrate N excretion rates were correlated with individual body mass. Additionally both mean N excretion rate per individual and per unit of biomass varied by nearly three orders of magnitude across invertebrate taxa. The invertebrate community supplied about 13% of nutrient demand. Nutrient supply rate by macroinvertebrates was dominated by the mayfly Baetis bicaudatus, Chironimidae, and the caddisfly Arctopsyche grandis. I also found that taxa with high mass-specific excretion rates tend to be most tolerant to organic pollution. This result could indicate the presence of a positive feedback chain where an initial disturbance leads to continued change in the invertebrate community. I determined that macroinvertebrates do play an important role in nutrient cycling in this Rocky Mountain stream and that changes in the macroinvertebrate community could have potentially large effects on stream nutrient cycling. 

Data variables arrangement: columns
Data variables description: Each row contains the length or width of a section of the East River.

Project Audit
Have these data been audited? No
Who audited these data? 
Are the metadata and associated data file of sufficient quality to repeat the study?  

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